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KMID : 0525720210260040187
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Journal of Chitin and Chitosan
2021 Volume.26 No. 4 p.187 ~ p.192
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Characterization of Starch Hydrolysis of Amylase from Paenibacillus peoriae KP-1
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Kim Sang-Jin
Choi Min-Su
Yu Sang-Mi
Park Chang-Su
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Abstract
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We screened amylase-producing microorganisms from environmental soil isolated from Mungyeong-si, Gyeongbuk, Korea. As a result, we selected one strain showing the highest amylase activity based on LB agar plate containing 1% starch and 0.02% trypan blue. In the analysis of 16S rRNA gene sequence from the strain, the isolated strain showed 99% of identity with Paenibacillus peoriae, therefore we named the strain Paenibacillus peoriae KP-1. When P. peoriae KP-1 was cultivated with LB medium with 0.5% starch, the strain produced amylase maximally after 5 days of cultivation. The amylase from P. peoriae KP-1 showed the highest activity at pH 7.0 and 50℃ and was stable up to 50℃ and at range of pH 6.0-8.0 against 1 hour of treatment. In the hydrolysis of starch, the amylase from P. peoriae KP-1 exhibited the highest activity in the 40 g/L of starch concentration. And, under the condition (pH 7.0, 40℃, and 40 g/L starch), the enzyme produced 30 g/L of reducing sugar after reaction for 300 min.
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KEYWORD
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Amylase, Characterization, Paenibacillus polymyxa, Soil, Starch
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